Crystallization of Nucleic Acids and Proteins: A Practical Approach(210 Practical Approach Series)

Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucliec Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocols to provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucliec Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology, crystallography, protein engineering, and biological chemistry.

Table of Contents:
Preface Giegé and Ducruix: An introduction to the crystallogenesis of biological macromolecules Lorber and Giegé: Biochemical aspects and handling of macromolecular solutions and crystals Berne, Doublie and Carter, Jr: Molecular biology for structural biology Carter, Jr: Experimental design, quantitive analysis, and the cartography of crystal growth Ducruix and Giegé: Methods of crystallization Robert et al: Crystallization in gels and related methods Stura: Seeding techniques Dock-Bregeon, Moras and Giegé: Nucleic acids and their complexes Réiss-Husson and Picot: Crystallization of membrane proteins Ries-Kaut and Ducruix: From solution to crystals with a physico-chemical aspect Veesler and Boistelle: Diagnostic of prenucleation and nucleation by spectroscopic methods and background on the physics Brisson, Lambert and Bergsma-Schutter: Two-dimensional crystallization of soluble proteins on planar lipid films Stura and Gleichmann: Soaking techniques Sawyer and Turner: X-ray analysis Index