DNA Cloning 4: A Practical Approach
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DNA Cloning 4: A Practical Approach: Mammalian Systems(164 Practical Approach Series)

DNA Cloning 4: A Practical Approach: Mammalian Systems(164 Practical Approach Series)


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About the Book

DNA cloning techniques have made their impact to most areas of biological research. This is the fourth and last volume in this set, offering a complete update and extension into new areas of David Glover's original DNA Cloning volumes. This volume describes a variety of techniques for genetic manipulation of mammalian cells. Topics covered include construction of Vaccinia virus recombinants, retroviral vectors, production of transgenic animals, expression using the HSV-1 vector system, and adenovirus vectors. The information presented is an up-to-date account of each research area by established researchers. The book includes detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments. This new 4-part companion volume set of Practical Approach titles aims to cover the major techniques required by all modern molecular biology laboratories.

Table of Contents:
1: High efficiency gene transfer into mammalian cells 2: Construction and characterization of Vaccinia virus recombinants 3: Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells 4: Retroviral vectors 5: Genetic manipulation of embryonic stem cells 6: Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs 7: Genomic and expression analysis of transgenic animals 8: Expression using a defective Herpes simplex virus (HSV-1) vector system 9: Adenovirus vectors


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Product Details
  • ISBN-13: 9780199634804
  • Publisher: Oxford University Press
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Spine Width: 17 mm
  • Weight: 514 gr
  • ISBN-10: 0199634807
  • Publisher Date: 25 Jan 1996
  • Height: 234 mm
  • No of Pages: 340
  • Series Title: 164 Practical Approach Series
  • Sub Title: Mammalian Systems
  • Width: 157 mm


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DNA Cloning 4: A Practical Approach: Mammalian Systems(164 Practical Approach Series)
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DNA Cloning 4: A Practical Approach: Mammalian Systems(164 Practical Approach Series)
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