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Cell-cell Interactions

Cell-cell Interactions


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About the Book

The type of communication between a cell and its immediate environment defines, to a large extent, the phenotype of that cell; it also determines the structure and function of the tissues and organs of which the cell is a part. This book describes the methods essential to the identification and characterization of the molecules that mediate cellular interactions in a variety of systems. Included are methods for preparing relevant probes, analyzing adhesive and junctional interactions in multicellular organizations, determining which structural features of a relevant molecule are functionally important, and for assaying the consequences of cell-cell interactions in cell culture. "Cell-cell Culture" aims to provide practical information relating to this area of cell biology. This book should therefore be useful to a range of biological scientists wishing to investigate the mechanisms and consequences of cellular interactions in their system of choice.



Table of Contents:
Assays of cell adhesion, S. Hofman; generation and characterization of function-blocking anti-cell adhesion molecule antibodies, Ann Acheson and Warren Gallin; expression cloning - transient expression and rescue from mammalian cells, Alejandro Aroffo; functional analysis of uvomorulin by site-directed mutagenesis and gene transfer into eukaryotic cells, Jorg Stappert and Rolf Kemler; integrin mutagenesis, A. Reszka and A. Horwitz; isolation of intercellular junctions, Elliot L. Hertzberg et al; the "Xenopus" oocyte cell-cell channel assay for functional analysis of gap junction proteins, Gerhard Dahl; patch-clamp analysis of gap junction currents, R.D. Veenstra and P.R. Brink; biochemical approaches for analyzing "de novo" assembly of epithelial junctional components, Manijeh Pasdar; biochemical methods for studying supramolecular complexes involving cell adhesion molecules, integral membrane proteins, and the cytoskeleton, W. James Nelson et al; electrophysiological assessment of epithelia, Karl J. Karnaky, Jr; compartmented culture analysis of nerve growth, Robert B. Campenot.


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Product Details
  • ISBN-13: 9780199633197
  • Publisher: Oxford University Press
  • Publisher Imprint: Oxford University Press
  • Language: English
  • ISBN-10: 0199633193
  • Publisher Date: 01 Nov 1992
  • Binding: Paperback
  • Returnable: N


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