Receptor Biochemistry
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Receptor Biochemistry: A Practical Approach(A Practical Approach)

Receptor Biochemistry: A Practical Approach(A Practical Approach)


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About the Book

This book is one of a trio of volumes covering practical aspects of neurotransmitter and hormone-receptor-ligand interactions, receptor biochemistry and receptor-effector coupling. The acquisition of 3-dimensional structural information about receptors, particularly through the application of crystallographic and other physical techniques, will require the purification and handling of significant amounts of receptor proteins. This book focuses particularly on strategies which have proved successful in the purification of a number of receptors of pharmacological importance. Included are examples of G-protein coupled receptors, oligomeric receptors which incorporate an integral ion channel, and receptor tyrosine kinases. Specific aspects covered include the choice of detergents for solubilization, the synthesis and utilization of specific affinity gels, the characterization of purified receptor proteins and techniques for specific polypeptide cleavage, peptide mapping and the isolation of sequenceable peptides. There is also an introduction to structural prediction from receptor sequences, and the high-level expression of cloned receptor genes. The book draws on the practical experience and insight of a number of scientists who have been at the forefront of progress in this difficult and demanding field.

Table of Contents:
T. Haga, & K. Haga: Solubilisation, purification and molecular characterisation of receptors: principles and strategy; K. Haga, & T. Haga: Purification of the muscarinic acetylcholine receptor from brain; A. Stephenson: Purification of the GABA/benzodiazepine receptor; P.G. Strange, & R.A. Williamson: Dopamine receptors: isolation and molecular characterisation; C. Demoliou-Mason, & E. Barnard: Purification of opioid receptors; J. Regan: Purification of B-adrenergic receptors; G. Panayotou, & M. Gregoriou: Purification of the epidermal growth factor receptor from A431 cell; M. Wheatley: Peptide mapping and sequencing and the generation and isolation of sequencable peptides from receptors; C. Fraser: Expression of receptor genes in cultured cells; M. Green: Structural deductions from receptor sequences.


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Product Details
  • ISBN-13: 9780199630929
  • Publisher: Oxford University Press
  • Binding: Hardback
  • Language: English
  • Sub Title: A Practical Approach
  • Width: 155 mm
  • ISBN-10: 0199630925
  • Publisher Date: /09/1990
  • Height: 235 mm
  • Series Title: A Practical Approach
  • Weight: 756 gr


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