The Initiation of DNA Replication
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The Initiation of DNA Replication

The Initiation of DNA Replication


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About the Book

The Initiation of DNA Replication contains the proceedings of the 1981 ICN-UCLA Symposia on Structure and DNA-Protein Interactions of Replication Origins, held in Salt Lake City, Utah on March 8-13, 1981. The papers explore the initiation of DNA replication and address relevant topics such as whether there are specific protein recognition sites within an origin; how many proteins interact at an origin and whether they interact in a specific temporal sequence; or whether origins can be subdivided into distinct functional domains. The specific biochemical steps in DNA chain initiation and how they are catalyzed are also discussed. This book is organized into six sections and comprised of 41 chapters. The discussion begins by analyzing the replication origin region of the Escherichia coli chromosome and the precise location of the region carrying autonomous replicating function. A genetic map of the replication and incompatibility regions of the resistance plasmids R100 and R1 is described, and several gene products produced in vivo or in vitro from the replication region are considered. The sections that follow focus on the DNA initiation determinants of bacteriophage M13 and of chimeric derivatives carrying foreign replication determinants; suppressor loci in E. coli; and enzymes and proteins involved in initiation of phage and bacterial chromosomes. The final chapters examine the origins of eukaryotic replication. This book will be of interest to scientists, students, and researchers in fields ranging from microbiology and molecular biology to biochemistry, molecular genetics, and physiology.

Table of Contents:
Contributors Preface I. Bacterial Replication Origins 1. Escherichia coli Origin of Replication: Structural Organization of the Region Essential for Autonomous Replication and the Recognition Frame Model 2. The DNA Replication Origin Region of the Enterobacteriaceae 3. Appendix: The Consensus Sequence of the Bacterial Origin 4. Mapping of Promoters in the Replication Origin Region of the E. coli Chromosome 5. Transcription and Translation Events in the oriC Region of the E. coli Chromosome 6. Genes, Transcriptional Units, and Functional Sites in and around the E. coli Replication Origin 7. Specific Binding of Escherichia coli Replicative Origin DNA to Membrane Preparations II. Plasmid Replication Origins 8. Dissection of the Replication Region Controlling Incompatibility, Copy Number, and Initiation of DNA Synthesis in the Resistance Plasmids, R100, and R1 9. Direct Repeats of Nucleotide Sequences are Involved in Plasmid Replication and Incompatibility 10. Incompatibility of IncFII R Plasmid NR1 11. Genetic Analysis of pMB1 Replication 12. Cloning of DNA Synthesis Initiation Determinants of ColE1 into Single-Stranded DNA Phages III. Bacteriophage Replication Origins 13. DNA Initiation Determinants of Bacteriophage M13 and of Chimeric Derivatives Carrying Foreign Replication Determinants 14. Essential Features of the Origin of Bacteriophage ?X174 RF DNA Replication: A Recognition and a Key Sequence for FX Gene A Protein 15. Viral DNA Sequences and Proteins Important in the ?X174 DNA Synthesis 16. Conservation and Divergence in Single-Stranded Phage DNA Secondary Structure: Relations to Origins of DNA Replication 17. Primary and Secondary Replication Signals in Bacteriophage ? and IS5 Insertion Element Initiation Systems 18. Interaction of Bacteriophage Lambda O Protein with the Lambda Origin Sequence 19. On the Role of Recombination and Topoisomerase in Primary and Secondary Initiation of T4 DNA Replication IV. Initiation Genes in Bacteria 20. Analysis of Seven dnaA Suppressor Loci in Escherichia coli 21. Suppression of Amber Mutations in the dnaA Gene of Escherichia coli K-12 by Secondary Mutations in rpoB 22. Evidence That the Escherichia coli DNAZ Product, a Polymerization Protein, Interacts In Vivo with the dnaA Product, an Initiation Protein 23. REC-Dependent DNA Replication in E. coli: Interaction between REC-Dependent and Normal DNA Replication Genes 24. An Alternative DNA Initiation Pathway in E. coli 25. Genetic Analysis of DNA Replication in Salmonella typhimurium V. Enzymes and Proteins in Initiation of Phage and Bacterial Chromosomes 26. Initiation of Bacteriophage T7 DNA Replication 27. The Primosome in ?X 174 Replication 28. Role of the ß Subunit of the Escherichia coli DNA Polymerase III Holoenzyme in the Initiation of DNA Elongation 29. Priming of Phage ?29 Replication by Protein p3, Covalently Linked to the 5' Ends of the DNA VI. Eukaryotic Replications Origins 30. Functional Components of the Saccharomyces cerevisiae Chromosomes—Replication Origins and Centromeric Sequences 31. DNA Sequences That Allow the Replication and Segregation of Yeast Chromosomes 32. Sequencing and Subcloning Analysis of Autonomously Replicating Sequences from Yeast Chromosomal DNA 33. Replication Origins Used In Vivo in Yeast 34. Replication Properties of Trp1-R1-Circle: A High Copy Number Yeast Chromosomal DNA Plasmid 35. In Vitro Replication of Yeast 2-µm Plasmid DNA 36. Replication in Monkey Cells of Plasmid DNA Containing the Minimal SV40 Origin 37. Locations of 5'-Ends of Nascent DNA at the Origin of Simian Virus 40 DNA Replication 38. Replication Directed by a Cloned Adenovirus Origin 39. Eukaryotic Origins: Studies of a Cloned Segment from Xenopus laevis and Comparisons with Human Blur Clones 40. Regions of Tetrahymena rDNA Allowing Autonomous Replication of Plasmids in Yeast 41. High-Frequency Transformation of Mouse L Cells Index


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Product Details
  • ISBN-13: 9780125835800
  • Publisher: Elsevier Science Publishing Co Inc
  • Publisher Imprint: Academic Press Inc
  • Language: English
  • Weight: 450 gr
  • ISBN-10: 0125835809
  • Publisher Date: 17 Mar 1982
  • Binding: Hardback
  • No of Pages: 628


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