Techniques in Confocal Microscopy: (Reliable Lab Solutions)

As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.

Table of Contents:
Part I. Practical Considerations and Equipment 1. Theoretical Basis of Confocal Microscopy Robert H. Webb 2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope Hao He, Rose Chik Ying Ong, Kam Tai Chan, Ho Pui Ho, Siu Kai Kong 3. Equipment for Mass Storage and Processing of Data Guy Cox 4. Antifading Agents for Confocal Fluorescence Microscopy Miguel Berrios, Kimberly A. Conlon, and David E. Colflesh 5. Mounting Techniques for Confocal Microscopy Manabu Kagayama and Yasuyuki Sasano 6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy Hisashi Hashimoto, Hiroshi Ishikawa, and Moriaki Kusakabe 7. Use of Confocal Microscopy to Investigate Cell Structure and Function Ghassan Bkaily, Danielle Jacques 8. Combining Laser Scanning Confocal Microscopy and Electron Microscopy Xuejun Sun Part II. Functional Approaches 9. Volume Measurements in Confocal Microscopy Carlos B. Mantilla, Y.S. Prakash and Gary C. Sieck 10. Quantitation of Phagocytosis by Confocal Microscopy George F. Babcock and Chad T Robinson MS 11. Receptor-Ligand Internalization Guido Orlandini, Nicoletta Ronda, Rita Gatti, Gian Carlo Gazzola, and Alberico Borghetti 12. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy David W. Piston and Susan M. Knobel 13. Trafficking of the Androgen Receptor Virginie Georget, Béatrice Terouanne, Jean-Claude Nicolas, and Charles Sultan 14. Localization of Proteases and Peptide Receptors by Confocal Microscopy Kanti D. Bhoola, Celia J. Snyman and Carlos D. Figueroa 15. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo Barry R. Masters, Peter T. C. So, Ki Hean Kim, Christof Buehler, and Enrico Gratton 16. Video-Rate, Scannng Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the Eye Barry R. Masters and Matthias Böhnke 17. In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) Method Sylvia Prilloff, Petra Henrich-Noack, Ralf Engelmann, Bernhard A. Sabel 18. Identification of Viral Infection by Confocal Microscopy David N. Howell and Sara E. Miller 19. Membrane Trafficking Sabine Kupzig, San San Lee, and George Banting Part III. Green Fluorescent Protein 20. Monitoring of Protein Secretion with Green Fluorescent Protein Christoph Kaether and Hans-Hermann Gerdes 21. Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters Xiaoning Zhao, Tommy Duong, Chiao-Chian Huang, Steven R. Kain, and Xianqiang Li 22. Measuring Protein Degradation with Green Fluorescent Protein Stephen R. Cronin and Randolph Y. Hampton 23. Studying Nuclear Receptors with Green Fluorescent Protein Fusions Gordon L. Hager 24. Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Protein Conjugates Larry S. Barak, Jie Zhang, Stephen S. G. Ferguson, Stephane A. Laporte, and Marc G. Caron 25. Fluorescent Proteins in Single- and Multicolor Flow Cytometry Lonnie Lybarger and Robert Chervenak 26. Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second-Messenger Signaling in Neurons L. A. C. Blair, K. K.Bence, and J. Marshall 27. Expression of Green Fluorescent Protein and Inositol 1,4,5-Triphosphate Receptor in Xenopus laevis Oocytes Atshushi Miyawaki, Julie M. Matheson, Lee G. Sayers, Akira Muto, Takayuki Michikawa, Teiichi Furuichi, and Katsuhiko Mikoshiba 28. Confocal Imaging of Ca2+, pH, Electrical Potential, and Membrane Permeability in Single Living Cells John J. Lemasters, Donna R. Trollinger, Ting Qian, Wayne E. Cascio, and Hisayuki Ohata Part IV. Laser Capture Microdissection 29. Laser Capture Microdissection and Its Applications in Genomics and Proteomics James L. Wittliff 30. Going in Vivo with Laser Microdissection Anette Mayer, Monika Stich, Dieter Brocksch, Karin Schütze, and Georgia Lahr 31. Flourescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections Douglas J. Demetrick, Sabita K. Murthy, and Lisa M. DiFrancesco

About the Author :
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science.