About the Book
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.
Table of Contents:
Volume 1; List of Contributors. Preface. Contents of Other Volumes. The Development and Application of Colloidal Gold as a Microscopic Probe, D.A. Handley. Methods for Synthesis of Colloidal Gold, D.A. Handley. Protein A-Gold and Protein G-Gold Postembedding Immunoelectron Microscopy, M. Bendayan. Preparation and Application of Lectin-Gold Complexes, N. Benhamou. Colloidal Gold for Multiple Staining, J. Doerr-Schott. Immunogold Labeling of Ultrathin Cryosections, P.M.P. van Bergen en Henegouwen. Colloidal Gold for Scanning Electron Microscopy, M. Horisberger. Backscattered Electron Imaging of the Colloidal Gold Marker on Cell Surfaces, E. De Harven and D. Soligo. Silver-Enhanced Colloidal Gold Method, L. Scopsi. Silver-Enhanced Colloidal Gold for Light Microscopy, G.W. Hacker. Strategies in Colloidal Gold Labeling of Cell Surfaces and Cytoskeletal Elements of Cultured Cells, G.B. Birrell and O. Hayes Griffith. Colloidal Gold-Immunoreplica Method, M.V. Nermut and A. Nicol. Immunogold Double-Diffusion Method, J.R. Harris. Colloidal Gold-Labeled Agarose-Gelatin Microspherule MethodK.-X. Gao. Colloidal Gold for Microbiological Immunocytochemistry, J.E. Beesley. Immunogold Labeling of Viruses in Suspension, E. Kjeldsberg. Plasma Membrane Localization of Proteins with Gold Immunocytochemistry, A.N. van den Pol, M. Ellisman, and T. Deerinck. Factors Affecting the Staining with Colloidal Gold, K. Park, H. Park, and R.M. Albrecht. Each chapter includes references. Index. Volume 2; List of Contributors. Preface. Contents of Other Volumes. Molecular Interactions between Colloidal Gold, Proteins, and Living Cells, P. Baudhuin, P. Van der Smissen, S. Beauvois, and P.J. Courtoy. Protein A-Gold: Nonspecific Binding and Cross-Contamination, A.D. Hyatt. Role of Tissue Processing in Colloidal Gold Methods, G.R. Newman and J.A. Hobot. LR White Embedding Medium for Colloidal Gold Methods, G.R. Newman. Lowicryls and Low-Temperature Embedding for Colloidal Gold Methods, J.A. Hobot. The Enzyme-Gold Cytochemical Approach: A Review, M. Bendayan. Preparation and Application of Lipoprotein-Gold Complex, D.A. Handley and C.M. Arbeeny. Preparation and Application of Albumin-Gold Complex, IS. Villaschi. Label-Fracture Cytochemistry, F.W.K. Kan and P.P. da Silva. Colloidal Gold Conjugates for Retrograde Neuronal Tracing, D. Menetrey and A.I. Basbaum. Colloidal Gold Labeling of Microtubules in Cleaved Whole Mounts of Cells, J.A. Traas. Colloidal Gold: Immunonegative Staining Method, J.E. Beesley. Immunogold Labeling of Viruses in Situ, S.M. Pietschmann, E.H.S. Hausmann and H.R. Gelderblom. Study of Exocytosis with Colloidal Gold and Other Methods, P. Buma.; Colloidal Gold Labeling of Acrylic Resin-Embedded Plant Tissues, E.M. Herman. Preembedding Immunogold Staining of Cell Surface-Associated Antigens Performed on Suspended Cells and Tissue Sections, C. Ferrari, G. De Panfilis, and G.C. Manara. Colloidal Gold in High-Voltage Electron Microscopy-Ruthenium Red Method and Whole-Cell Mount, K. Takata and H. Hirano. Correlative Light and Electron Microscopic Immunocytochemistry in Reembedded Resin Sections with Colloidal Gold, H. Mar and T.N. Wight. Streptavidin-Gold Labeling for Ultrastructural in Situ Nucleic Acid Hybridization, R.A. Wolber and T.F. Beals. Detection of Proteins with Colloidal Gold, R. Rohringer. Undecagold-Antibody Method, J.F. Hainfeld. Immunogold Labeling for the Single-Laser FACS Analysis of Triple Antibody-Binding Cells, T.H. Totterman and R. Festin. Silver-Enhanced Colloidal Gold for the Detection of Leukocyte Cell Surface Antigens in Dark-Field and Epipolarization Microscopy, M. De Waele. Each chapter includes references. Index. Volume 3; Contributors. Preface. Contents of Other Volumes. Some Basic Concepts for the Choice of Methods, E. Kellenberger and M.A. Hayat. Techniques for the Production of Monoclonal and Polyclonal Antibodies, K.J. Morrow, Jr., E. Unuvar, S.W. King, and J.B. Mleczko. Lowicryl Resins, W. Villiger. Polar or Apolar Lowicryl Resin for Immunolabeling?, M. Durrenberger, W. Villiger, B. Arnold, B.M. Humble and S. Schwarz. Yield of Immunolabel Compared to Resin Sections and Thawed Cryosections, Y.-D. Stierhof, H. Schwarz, M. Durrenburger, W. Villiger and E. Kellenberger. Quantitation of Colloidal Gold by Electron Microscopy, T. Kehle and V. Herzog. Colloidal Gold Labeling for Deteremining Cell Surface Area, V. Herzog and T. Kehle. Simultaneous Demonstration of Antigens on Outer and Protoplasmic Surfaces of the Plasma Membrane by Replica Immunocytochemistry, G. Rutter and H. Hohenberg. Double Labeling of Antigenic Sites on Cell Surfaces Imaged with Backscattered Electrons, E. Namork. Location and Identification of Colloidal Gold Particles with an Energy Dispersive Analyzer, S. Eskelinen and R. Peura. Use of Dinitrophenol IgG Conjugates: Immunogold Labeling of Cellular Antigens on Thin Sections of Osmicated and Epon-Embedded Specimens, R. Pathak and R.G.W. Anderson. Preparation and Application of Insulin-Gold Complex, R.M. Smith and L. Jarett. Microinjection of Colloidal Gold, S.I. Dworetzky. Combined Diaminobenzidine-Colloidal Gold Staining, H.C.F. Mutasa. Colloidal Gold Techniques and the Immunocytochemistry of Antigen-Antibody Reactions at Interfaces, H. Nygren and M. Werthen. Colloidal Gold Labeling for Diagnostic Pathology, G.A. Herrara and R.L. Lott. Immunogold Silver Staining Using Microwave Irradiation, M.E. Boon, H.J.G. van de Kant, and L.P. Kok. A Correlative Approach to Colloidal Gold Labeling with Video-Enhanced Light Microscopy, Low Voltage Scanning Electron Microscopy, and High Voltage Electron Microscopy, S.L. Goodman, K. Park, and R.M. Albrecht. Index.
Review :
The chapters in these volumes are a measure of a healthy field of research and its applications. --ELECTRON MICROSCOPY REVIEW If you need to localise something within a cell, the colloidal gold series can probably tell you how to go about it. --EUROPEAN JOURNAL OF PROTISTOL This treatise is timely and extremely helpful for the scientist interested in applying the colloidal gold methodology for a particular research problem... The present volumes elegantly cover broad research applications that reach beyond the traditional field of microscopy in cell biology and extend into the field of molecular biology... An attempt has been made to include a distinguished group of experts who are the originators of the techniques; many continue to play an active role in the technical development. The authors are from diverse fields, so that these books will be attractive to a wide audience including cell biologists, pathologists, virologists, and developmental and molecular biologists. The printing of the hardbound books is of prime quality, with excellent reproductions of the well-chosen micrographs... The text, printed on glossy paper, is extremely easy to read; diagrams, drawings, and tables throughout the two volumes are particularly pertinent in illustrating the principles and steps of many of the procedures. Although each chapter is written by a different author, all consistently follow a well organized pattern. The chapters begin with an introduction and historical background, then follow with preparation of probes and specimens, principles and descriptions of the methods for labeling, descriptions of the experimental results with a review of the literature, conclusions, and a list of citations to many original references. A particularly valuable part of each chapter is the assessment of the advantages, limitation, and pitfalls of a given methodology... These two volumes are a mix of reference book, student textbook, and cookbook. On the whole, the descriptions of methods are thorough and stimulating, and will be most useful to the experienced reader seeking to select the procedure most suited to a particular research problem. The in-depth account of the methodology and the extensive references provided will be of great help to those interested in assessing the potential and limitations of colloidal gold methodology. These books are a good blend of the old and the new, and are at their best when they provide a focused presentation of the recent direction in the field of the red colloid. --EMSA BULLETIN
