Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas
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Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas: Molecular Genetics: Liver and Pancreatic Carcinomas V3

Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas: Molecular Genetics: Liver and Pancreatic Carcinomas V3


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About the Book

Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today.

Table of Contents:
Chapter 1: Quality Assurance in Immunohistochemistry Chapter 2: Methodology of Microarray Data Analysis Chapter 3: Microarray-Based Gene Expression Analysis of Defined Tumor Areas Chapter 4: Tissue Microarray and Quantitative Fluorescence Image Analysis in Tumor Biomarker Analysis Chapter 5: Identification of Differentially Expressed Genes Using Rapid Subtraction Hybridization: Detailed Methodology for Performing Rapid Subtraction Hybridization Chapter 6: Noncontact Laser Microdissection and Pressure Catapulting: A Basic Tool in Genomics, Transcriptomics, and Proteomics Chapter 7: DNA Methylation Analysis of Human Cancer Chapter 8: Antiepidermal Growth Factor Receptor Antibody: Immunohistochemistry Chapter 9: PTEN and Cancer Chapter 10: The Role of p53 and p73 Genes in Tumor Formation Chapter 11: Analysis of Centrosome Amplification in Cancer Chapter 1: Liver Carcinoma Chapter 2: Mechanisms of Human Hepatocarcinogenesis: An Overview Chapter 3: Genes Involved in Hepatocellular Carcinoma Chapter 4: Role of p53 and ZBP-89 in Hepatocellular Carcinoma Chapter 5: Role of Immunohistochemical Expression of PTEN in Hepatocellular Carcinoma Chapter 6: The Potential Role of Bcl-2 and Protein Expression in Hepatocellular Carcinomas: A Clinicopathologic Study Chapter 7: The Detection and Clinical Aspect of Immunohistochemical Analysis of Bcl-xL Protein in Hepatocellular Carcinoma Chapter 8: Growth Arrest DNA Damage-Inducible Gene 45 β in Human Hepatocellular Carcinoma Chapter 9: Immunohistochemical Detection of DNA Topoisomerase IIα in Hepatocellular Carcinoma Chapter 10: Role of Immunohistochemical Expression of DNA Methyltransferases in Hepatocellular Carcinoma Chapter 11: Role of Hepatitis B Surface Antigen in Hepatocarcinogenesis Chapter 12: Immunohistochemical Detection of Host Gene Products Up-Regulated by Hepatitis B Virus X Antigen and Their Putative Roles in Hepatocellular Carcinoma Chapter 13: Distinction between Well-Differentiated Hepatocellular Carcinoma and Benign Hepatocellular Nodular Lesions, Using Fine-Needle Aspiration Biopsy Chapter 14: Immunohistochemical Detection of EBAG9/RCAS1 Expression in Hepatocellular Carcinoma Chapter 15: Hepatocellular Carcinoma of Differential Viral Origin: Analysis by Oligonucleotide Microarray Chapter 1: Pancreatic Carcinoma: An Introduction Chapter 2: Detection of Genomic Imbalances in Endocrine Pancreatic Tumors Using Comparative Genomic Hybridization Chapter 3: Molecular Detection of Micrometastases in Pancreatic Cancer Chapter 4: K-ras Protooncogene in Human Pancreatic Cancer Chapter 5: Altered Expression of p27 Protein in Pancreatic Carcinoma Chapter 6: The Role of DUSP6/MKP-3 in Pancreatic Carcinoma Chapter 7: Expression of Carbohydrate Antigens in Pancreatic Cancer Chapter 8: Role of Mucins in Pancreatic Carcinoma Chapter 9: Immunohistochemical Detection of Cyclooxygenase-2 in Pancreatic Ductal Adenocarcinomas and Neuroendocrine Tumors Chapter 10: Immunohistochemical Detection of Activated Stat3 Protein in Pancreatic Cancer Chapter 11: The Role of Transcription Factor E2F-1 in Pancreatic Ductal Chapter 12: Role of Immunohistochemical Expression of Chromogranins in Pancreatic Endocrine Tumors Chapter 13: Role of Immunohistochemical Expression of Maspin in Pancreatic Carcinoma Chapter 14: Role of Mast Cells in Pancreatic Carcinoma Chapter 15: Endocrine Cells in Invasive Ductal Adenocarcinoma of the Pancreas: An Immunohistochemical Study Chapter 16: Fine-Needle Aspiration Biopsy of Pancreatic Adenocarcinoma Chapter 17: Immunohistochemical Expression of Mitogen-Activated Protein Kinase Kinase 2 in Pancreatic Cancer Index

About the Author :
Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.

Review :
"...this book contains a wealth of information of value to the oncologist and biomedical scientist." --MICRON (2005) “Professor Hayat’s Handbook has the great potential of becoming the handbook of choice for clinician and basic scientists.” -–Akhouri A. Sinha, Ph.D., Professor of Genetics, University of Minnesota and Research Scientist at the VA Medical Center


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Product Details
  • ISBN-13: 9780120884049
  • Publisher: Elsevier Science Publishing Co Inc
  • Publisher Imprint: Academic Press Inc
  • Height: 279 mm
  • No of Pages: 432
  • Weight: 1840 gr
  • ISBN-10: 0120884046
  • Publisher Date: 21 Sep 2005
  • Binding: Hardback
  • Language: English
  • Sub Title: Molecular Genetics: Liver and Pancreatic Carcinomas V3
  • Width: 216 mm


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Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas: Molecular Genetics: Liver and Pancreatic Carcinomas V3
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Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas: Molecular Genetics: Liver and Pancreatic Carcinomas V3
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