Laboratory Exercises in Organismal and Molecular Microbiology

A modern general microbiology laboratory manual that combines the procedural details of a laboratory manual with the photographic support of a laboratory atlas. The 46 class-tested laboratory experiments are divided into 9 specialty areas, and the extensive four-color illustration program includes 220 photos and micrographs plus 150 line drawings.

Table of Contents:
Safety in the Microbiology LaboratoryI Survey of Microscopic OrganismsA The Microscope1 Structure, Function, and Use of the Microscope2 Microscopic Comparisons of Microorganisms, PMulticellular arasites, and Microscopic InvertebratesB Microorganisms3 Microbial Prokaryotes: Bacteria and Cyanobacteria 4 Microbial Eucaryotes: Fungi5 Microbial Eucaryotes: Protozoans and AlgaeC Multicellular Parasites6 Flatworms and RoundwormsD Microscopic Invertebrates7 Zooplankton8 Disease VectorsIIStaining TechniquesA Morphological Stains9 Negative Stain10 Smear Preparation and the Simple StainB Differential Stains11 Gram Stain12 Acid-fast StainC Structural Stains13 Spore Stain14 Capsule and Flagella StainsD Application15 The Staining Characterization of a Bacterial UnknownIIIBacterial CultivationA Aseptic Technique16 Bacteria and Fungi in the Laboratory Environment: The Necessity of Aseptic TechniqueB Culture of Bacteria17 Preparation and Inoculation of Growth Media18 Culture Characterization of BacteriaIV Bacterial Identification19 Biochemical Tests Used to Identify Bacteria20 Application: The Identification of a Nonclinical Bacterial UnknownV Medical MicrobiologyA Bacteria of the Human Body21 Isolation and Identification of Staphylococci from the Skin22 Isolation and Identification of Streptococci from the Throat23A Identification of Enteric Bacteria, Including the Intestinal Pathogens Salmonella and Shigella23B Enterotubes, a Rapid Test System to Identify Enteric Bacteria24 Isolation and Identification of Bacteria from the Urinary TractB Treatment of Bacterial Infections25 Assessing Antibiotic Effectiveness: The Kirby-Bauer MethodC Application26 Identification of a Clinical Bacterial UnknownVI Controlling the Risk and Spread of Bacterial InfectionsA Proper Food Handling27 Killing Bacteria with High TemperatureB Disinfecting Skin and Countertops28 Skin Disinfection: Evaluating Antiseptics and Hand Sanitizers29 Cleaning Countertops with DisinfectantsC Testing Drinking Water Safety30 Bacteriological Examination of Drinking Water Using the MPN MethodVIIBacterial GeneticsA Bacterial Genomic DNA31 Bacterial DNA Isolation and Southern Analysis32 Mutagenesis in Bacteria: The Ames TestB Plasmid DNA33 Plasmid Isolation and Restriction MappingC The Transfer of Drug Resistance34 Acquiring Antibiotic Resistance Through Bacterial Transformation35 Acquiring Antibiotic Resistance Through Bacterial ConjugationVIII VirusesA The Nature and Characterization of Viruses36 Viral DNA FingerprintingB Virus Propagation37 Isolation of Bacteriophage from Sewage and Determination of Phage Titer38 The Virus Infection Cycle: The One-step Growth Curve39 Infection of Plant Leaves with Tobacco Mosaic VirusIXHematology and SerologyA Hematology40 Identification and Enumeration of White Blood CellsB Serology41 Antigen-Antibody Precipitation Reactions and Determination of Antibody TiterC Serological Techniques42 Agglutination Reactions: ABO Blood Typing43 Immunodiffusion: Antigen-Antibody Precipitation Reactions in Gels44 Enzyme-Linked Immunosorbent Assay (ELISA)45 Bacterial Protein Fingerprinting and Western Blotting46 The Neutralization of Viruses by Antibodies