Microbiology Experiments: A Health Science Perspective

All experiments are correlated to "Nester's Microbiology: A Human Perspective, 4/e", but can be used with any non-majors/allied health microbiology textbook.

Table of Contents:
1 Basic Microbiology 1 Ubiquity of Microorganisms 2 Bright-field Light Microscopy, Including History and Working Principles 3 Microscopic (Bright-field and Dark-field) Determination of Cell Motility, Form, and Viability Using Wet Mount and Hanging Drop Preparations 4 Simple Stains: Positive and Negative Stains 5 Multiple and Differential Stains 6 Pure Culture and Aseptic Technique 7 Defined, Undefined, Selective and Differential Media 8 Quantification of Microorganisms 9 Aerobic and Anaerobic Growth 10 The Effect of Incubation Temperature on Generation Time 11 Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time 12 Control of Microbial Growth with Ultraviolet Light 13 Osmotic Pressure, and Its Effect on the Rate and Amount of Microbial Growth 14 Antiseptics and Antibiotics 15 Selection of Bacterial Mutants Resistant to Antibiotics 16 Transformation: A Form of Genetic Recombination 17 Bacterial Conjugation 18 Gene Regulation: Induction and Catabolite Repression 2 The Other Microbial World 19 Microscopic Identification of Fungi 20 Parasitology: Protozoa and Helminths 21 Prokaryotic Viruses: Bacteriophage Isolation and Titering 3 Microbes and Humans 22 Normal Skin Flora 23 Respiratory Microorganisms 24 Identification of Enteric Gram-Negative Rods 25 Clinical Unknown Identification 4 Immunology 26 Differential White Blood Cell Stains 27 Lysozyme, an Enzymatic Form of Natural Resistance 28 Traditional Agglutination Reactions Employing Microbial and Nonmicrobial Antigens 29 Lancefield Grouping of Pathogenic Streptococci with a Latex Slide Agglutination Test 30 Use of an Enzyme-Linked Immunosorbent Assay (ELISA) Test for Coccidioides immitis Identification 31 An Ouchterlony Double Immunodiffusion Test for Coccidioides immitis Identification 5 Public Health 32 Epidemiology: A Staphylococcus Carrier Study 33 Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques 6 Applications of Biotechnology 34 Identifying DNA with Restriction Enzymes 35 Identification of Bacteria Using the Ribosomal Data Project 7 Projects 36 Hydrocarbon-Degrading Bacteria, Cleaning Up After Oil Spills 37 Luminescent Bacteria: Bacteria That Produce Light 38 Methylotrophs, Organisms That Grow on One-Carbon Compounds 39 Deinococcus, Bacteria with Out-Of-This-World Capabilities Appendices 1 Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in this Manual 2 Dilution Practice Problems 3 Metric System, Use of with Conversions 4 pH Adjustment of Liquid and Agar Growth Media 5 Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size 6 Use of the Hemocytometer for Determining Total Cell Number in a Liquid Suspension 7 Preparation of Covered Slide Cultures for Study of the Intact Structure of a Mold Thallus