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Application of Molecular Cytogenetics Techniques in Cancer Research

Application of Molecular Cytogenetics Techniques in Cancer Research


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About the Book

This dissertation, "Application of Molecular Cytogenetics Techniques in Cancer Research" by Liang, Hu, 胡亮, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract Hu Liang Abstract of thesis entitled Application of Molecular Cytogenetics Techniques in Cancer Research Submitted by Hu Liang for the degree of Doctor of Philosophy at The University of Hong Kong in November 2003 Cytogenetics, the study of chromosomes, is a field of genetics which combines the skills of cytologists, who study the structure and activities of cells, and geneticists, who study the relationship between the mechanism of heredity and cellular activities. A recent branch of this science, molecular cytogenetics, deploys in situ hybridization- based technology to analyze genetic alterations. Chromosome banding techniques have enabled chromosomes to be identified and chromosome abnormalities to be described at a primary level. However, even with the advent of high-resolution banding technique (more than 1,000 band level), banding analysis cannot achieve a resolution better than 3 Mb of DNA. Its accuracy is also ii Abstract Hu Liang highly dependent on the skill and experience of the individual cytogeneticist. In cancer, since the aberration of chromosomes is very complicated, it is very difficult to decipher highly rearranged chromosomes on the basis of the interpretation of a monochrome banding pattern. A major technological advance in molecular cytogenetics was achieved with the development of fluorescence in situ hybridization (FISH) in the late 1980s. A number of related techniques have since been developed, including primed in situ labeling (PRINS), fiber FISH, comparative genomic hybridization (CGH), spectral karyotyping (SKY), multiple color FISH (M-FISH), color banding, FISH with multiple subtelomeric probes, and array-based CGH. These modern techniques allow the copy number change of oncogene and tumor suppressor genes in cancer cells to be assessed, and the complex rearrangement of chromosomes in cancer cells to be fully characterized. This thesis describes the results of experiments with molecular cytogenetic techniques (including micro-FISH, CGH, and M-FISH) and traditional chromosome banding techniques to study chromosome abnormalities in cancer cells. A complete set of 24 whole chromosome painting probes (WCP), 43 chromosome arm painting probes (CAP), and 43 terminal band probes (TBP) were generated, together with 25 region- specific probes. Molecular cytogenetic techniques were combined with Southern blot, Northern blot, cDNA microarray and tissue microarray, enabling a homogenously staining region (HSR) in a metastatic hepatocellular carcinoma (HCC) cell line and a iii Abstract Hu Liang pair of primary and metastatic HCC cell lines to be characterized. FGF3 and vimentin was found probably to play important roles in HCC metastasis. Tissue microarray showed that the rate of upregulation of FGF3 (19/40 cases) and overexpression of vimentin (24/53 cases) in metastatic HCC is much higher than that of primary HCC cases (3/40 cases and 6/53 cases, respectively) (pThe experiments demonstrated that molecular cytogenetics is a very useful tool for the study of chromosome rearrange


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Product Details
  • ISBN-13: 9781374728097
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • No of Pages: 96
  • Weight: 245 gr
  • ISBN-10: 1374728098
  • Publisher Date: 27 Jan 2017
  • Binding: Paperback
  • Language: English
  • Spine Width: 5 mm
  • Width: 216 mm


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