Buy Differential Effects of Glial Cell Line-Derived Neurotrophic Factor and Neurturin on Ng108-15 Cells
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
21%
Differential Effects of Glial Cell Line-Derived Neurotrophic Factor and Neurturin on Ng108-15 Cells

Differential Effects of Glial Cell Line-Derived Neurotrophic Factor and Neurturin on Ng108-15 Cells


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

This dissertation, "Differential Effects of Glial Cell Line-derived Neurotrophic Factor and Neurturin on NG108-15 Cells" by Hui-kwan, Rebecca, Lee, 李曉鈞, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled Differential Effects of Glial Cell Line-Derived Neurotrophic Factor and Neurturin on NG108-15 Cells Submitted by Lee Hui Kwan, Rebecca for the degree of Master of Philosophy at The University of Hong Kong in December 2003 The RET proto-oncogene encodes a receptor tyrosine kinase which is important for the development of the enteric nervous system and the kidney. Alternate splicing of RET generates three isoforms of 1072, 1106 and 1114 amino acids. The long isoform of 1114 amino acids, referred as RET51, contains 51 unique amino acids at the carboxyl terminus that are replaced by 43 amino acids in RET43 and nine residues in RET9. The RET9 and RET51 transcripts are relatively more abundant. RET activation is triggered by members of the glial cell line-derived neurotrophic factor (GDNF) family ligands (GFLs) consisting of GDNF, neurturin (NTN), artemin (ARTN) and persephin (PSP). High affinity binding of RET to its cognate ligands is mediated by complexing with a member of glycosyl-phosphatidylinositol linked protein termed GDNF family receptor alpha: GFRα1-4. Each GFLs forms a preferred ligand-coreceptor complex. GDNF preferably bind to GFRα1 while NTN, ARTN and PSP binds to GFRα2,3 and 4, respectively. To better understand the distinct signaling profiles generated by GDNF and NTN, a neuroblastoma/glioma hybrid cell line, NG108-15, was used in the present study. NG108-15 cells were found to express high levels of RET and GFRα1 but GFRα2 expression was below the level of detection. The ligand-coreceptor complex being investigated was therefore GDNF-GFRα1 and NTN-GFRα1. I found that RET9 and RET51 formed homodimers instead of heterodimers upon ligand stimulation by immunoprecipitation and western blotting. Intriguingly, RET51 proteins may be translocated into the nucleus after NTN stimulation from immunocytochemical studies. Tyrosine (Y) 905, 1015, 1062 and 1096 are the main tyrosine phosphorylation sites in RET. Both GDNF and NTN induced a rapid phosphorylation of these sites. Rapid and transient Erk1/2 activation was found in GDNF-treated cells. On the other hand, a rapid and sustained Erk1/2 activation was observed in NTN-treated cells. Phosphorylation of PLCγ induced by NTN was more prolonged and pronounced whereas a weaker PLCγ phosphorylation was induced by GDNF. For the biological responses, NTN but not GDNF stimulated differentiation through PLCγ activation. On the other hand, GDNF was found to act as a survival factor. There was no observable effect of GDNF and NTN on proliferation and cell migration. In order to identify different substrates of phosphorylation triggered by GDNF and NTN, mass spectrometry was used. Several phosphorylated proteins were identified in both untreated and NTN-treated cells. These included heat shock protein 90-beta, heat shock cognate 71kD protein and myosin heavy chain. Ribonucleoside-diphophate reductase M1 chain and stress-induced-phosphoprotein 1 were only found to be phosphorylated in untreated cells. In nuclear fraction of GDNF-treated cells, myc far upstream-binding protein was phosphorylated. The roles of these proteins in RET signaling remain to be determined. DOI: 10.5353/th_b2777136 Subjects: Cell differentiation Neurotrophic functions Cellular signal transduction


Best Sellers


Product Details
  • ISBN-13: 9781374712287
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • No of Pages: 132
  • Weight: 322 gr
  • ISBN-10: 1374712280
  • Publisher Date: 27 Jan 2017
  • Binding: Paperback
  • Language: English
  • Spine Width: 7 mm
  • Width: 216 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Differential Effects of Glial Cell Line-Derived Neurotrophic Factor and Neurturin on Ng108-15 Cells
Open Dissertation Press -
Differential Effects of Glial Cell Line-Derived Neurotrophic Factor and Neurturin on Ng108-15 Cells
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Differential Effects of Glial Cell Line-Derived Neurotrophic Factor and Neurturin on Ng108-15 Cells

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!