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Home > Society and Social Sciences > Politics and government > Determination of the (15n/14n) and (18o/16o) Nitrate in Solids: Rsil Lab Code 2897
Determination of the (15n/14n) and (18o/16o) Nitrate in Solids: Rsil Lab Code 2897

Determination of the (15n/14n) and (18o/16o) Nitrate in Solids: Rsil Lab Code 2897


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Summary of Procedure The purpose of Reston Stable Isotope Laboratory (RSIL) lab code 2897 is to determine the (15N/14N), abbreviated as 15N, and (18O/16O), abbreviated as (18O, of nitrate (NO3-) in solids. The NO3- fraction of the nitrogen species is dissolved by water (called leaching) and can be analyzed by the bacterial method covered in RSIL lab code 2900. After leaching, the 15N and 18O of dissolved NO3- is analyzed by conversion of NO3- to nitrous oxide (N2O), which serves as the analyte for mass spectrometry. A culture of denitrifying bacteria is used in the enzymatic conversion of NO3- to N2O, which follows the pathway shown in equation 1. Because the bacteria Pseudomonas aureofaciens lacks N2O reductive activity, the reaction stops at N2O, unlike the typical denitrification reaction, which goes to N2. After several hours, the conversion is complete, and the N2O is extracted from the vial, separated from water vapor by Nafion drier and from CO2 with a layered Mg(ClO4)2/Ascarite trap, and trapped in a small-volume trap immersed in liquid nitrogen. After the N2O is released, it is further purified by gas chromatography (GC) before introduction to the isotope-ratio mass spectrometer (IRMS). The IRMS is a Finnigan DeltaPlus continuous flow IRMS (CF-IRMS). It has a universal triple collector, consisting of two wide cups with a narrow cup in the middle; it is capable of simultaneously measuring mass/charge (m/z) of the N2O molecule 44, 45, and 46. The ion beams from these m/z values are as follows: m/z = 44 = N2O = 14N14N16O; m/z = 45 = N2O = 14N15N16O or 14N14N17O; m/z = 46 = N2O = 14N14N18O. The 17O contributions to the m/z 44 and m/z 45 ion beams are accounted for before 15N values are reported.


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Product Details
  • ISBN-13: 9781249144335
  • Publisher: Bibliogov
  • Publisher Imprint: Bibliogov
  • Height: 246 mm
  • Sub Title: Rsil Lab Code 2897
  • Width: 189 mm
  • ISBN-10: 1249144337
  • Publisher Date: 01 Jul 2012
  • Binding: Paperback
  • Spine Width: 2 mm
  • Weight: 86 gr


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Determination of the (15n/14n) and (18o/16o) Nitrate in Solids: Rsil Lab Code 2897
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