Life in the Frozen State

While it is barely 50 years since the first reliable reports of the recovery of living cells frozen to cryogenic temperatures, there has been tremendous growth in the use of cryobiology in medicine, agriculture, horticulture, forestry, and the conservation of endangered or economically important species. As the first major text on cryobiology in the genomic era, Life in the Frozen State describes the current understanding of how living cells and complex organisms survive very low temperatures. Leading world experts combine fundamental theory and practice across a spectrum of species and applications to evaluate how cryobiology can benefit humanity. Chapters encompass disciplines ranging from mathematical modeling and biophysics, to the molecular biology of stress gene expression and the clinical banking of cells and tissues. This book provides a unique opportunity to explore the subject in a multidisciplinary context, which has historically been the key to realizing some of the most exciting advances in low temperature research. Features Integrates fundamental theory and practice across a broad range of species and applications Discusses cryobiology within a multidisciplinary context Emphasizes how the current knowledge of cryobiology can be applied to benefit humanity through health care and conservation

Table of Contents:
FUNDAMENTAL ASPECTS Principles of Cryobiology, Peter Mazur The Water to Ice Transition - Implications for Living Cells, Ken Muldrew, Jason Acker, Gloria Elliott, and Locksley McGann LIFE AND DEATH AT LOW TEMPERATURES Life in the Polar Terrestrial Environment with a Focus on Algae and Cyanobacteria, Josef Elster and Erica Benson Microbial Life in Permafrost: Extended Time in Extreme Conditions, Monica Ponder, Tatiana Vishnivetskaya, John McGrath, and James Tiedje Adaptation of Higher Plants to Freezing, Roger Pearce Oxidative Stress in the Frozen Plant: A Free Radical Point of View, Erica Benson and David Bremmer Physiology, Biochemistry, and Molecular Biology of Vertebrate Freeze Tolerance: The Wood Frog, Kenneth B. Storey and Janet M. Storey FREEZING AND BANKING OF LIVING RESOURCES Preservation of Fungi and Yeasts, C. Shuhui Tan and Cor van Ingen Cryo-conserving Algal and Plant Diversity: Historical Perspectives and Future Challenges, Erica Benson Plant Cryopreservation, Akira Sakai The Early History of Gamete Cryobiology, Stanley P. Leibo Cryobiology of Gametes and the Breeding of Domestic Animals, Alban Massip, Stanley P. Leibo, and Elizabeth Blesbois Cryopreservation as a Supporting Measure in Species Conservation: "Not the Frozen Zoo", Amanda R. Pickford and William V. Holt Cryopreservation of Gametes and Embryos of Aquatic Species, Tiantian Zhang Fundamental Issues for Cell-line Banks in Biotechnology and Regulatory Affairs, Glyn Stacey MEDICAL APPLICATIONS Mechanisms of Injury Caused by in vivo Freezing, Nathan E. Hoffman and John C. Bischof Cryopreservation in Transfusion Medicine and Haematology, Andreas Sputtek and Rebekka Sputtek Cryopreservation of Human Gametes and Embryos, Barry Fuller, Sharon Paynter, and Paul Watson The Scientific Basis for Tissue Banking, Monica Wusteman and Charles J. Hunt Engineering Desiccation Tolerance in Mammalian Cells: Tools and Techniques, Jason P. Acker, Tani Chen, Alex Fowler, and Mehmet Toner Stabilization of Cells During Freeze-Drying: The Trehalose Myth, John H. Crowe, Lois M. Crowe, Fern Tablin, William Wolkers, Ann E. Oliver, and Nelly M. Tsvetkova Vitrification in Tissue Preservation: New Developments, Mike J. Taylor, Yin C. Song, and Kelvin G.M. Brockband THE FUTURE OF CRYOBIOLOGY The Future of Cryobiology, Nick Lane, Barry Fuller, and Erica Benson